FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19175131
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA354450.184848802336735No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG322580.16822831614553246No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG225490.11759502451378298No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG223090.11634340333841788No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC98050.060.4628229
TTCCGAC106450.055.779558
TTTCCGA114050.052.3122447
GTTTCCG103650.050.921896
TTCGACG8100.047.4976271
GCTCCGT80050.047.0526161
CTCCGTT92550.045.368042
CGTTTCC114750.045.1041345
TCCGTTT118100.043.395833
GTTCCGT31200.040.494771
TGTCGGG32100.040.383155
CTCGCCG83300.039.8861476
TCGACGT10750.039.3238222
TAGTAGC167750.038.5370037
GCCCCGT42950.037.7105751
TCGCTCG11750.037.5942573
GTAGCAA174000.037.5355459
CCGTTTC140800.037.1730464
GACCCGT15050.035.9782681
CCCGTTA28800.035.6235923
Produced by FastQC (version 0.11.5)