FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6659069
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG89810.13486870311750787No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA88060.1322407081230124No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA66200.045.9466445
GCAGCGC10850.045.5114258
ATCAACG70200.043.4981883
TCAACGC70450.043.428324
GTATCAA76050.042.6731641
CGCAGAG73000.041.64578
ACGCAGA73500.041.2808277
AACGCAG75250.040.3995676
GGTATCA34250.039.9875531
GCAGAGT93000.032.4342359
TCTGTCG10700.026.6888318
AGTACGG89650.024.02931612-13
GAGTACG81350.023.92066812-13
TATCAAC132100.023.7915122
GCTCTAG18850.023.6923141
ACGGGAC8800.023.6742533
TGGTCCT30300.023.1808995
AGAGTAC108350.021.91320610-11
CTAGCCT48550.020.9651414
TATACCG29100.020.8530456
Produced by FastQC (version 0.11.5)