FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18080114
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1250750.6917821425241013No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA681900.3771547015688065No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG675720.37373658152819167No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG567700.3139913830189345No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT503700.27859337612583635No Hit
GGGCCAGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA457880.2532506155658089No Hit
GGGCCTGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA388380.21481059245533518No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG354500.19607177255630137No Hit
GGGCTGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA323210.1787654657487226No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA304790.16857747688980282No Hit
GTTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG288680.15966713484218076No Hit
TGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGG263100.14551899396209558No Hit
GGGTCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA257770.14257100370052977No Hit
GGGTCAGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA253310.140104205095167No Hit
GGGCCCGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA242110.13390955388887482No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG239860.13266509270903934No Hit
GGTCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA217590.1203476924979566No Hit
GCTCCATTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG213360.11800810547986589No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT210410.11637647859963715No Hit
GGGCAGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA199580.1103864721206957No Hit
GGGCTAGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA198480.10977806887722058No Hit
TCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGA189680.10491084292941959No Hit
GGGACGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA183920.10172502230904074No Hit
GGGTTGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA182810.10111108812698857No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGG58900.063.4959375
ACCGGTT36900.062.765543
TTCCGAC475150.060.7636388
TCCGACC475150.060.7123459
GTCCGGT44450.059.899242
TTTCCGA502100.057.625067
GTTCCGT67200.056.159431
CTGTCGG65650.055.377874
GACGGTT35700.054.6388363
TCCGGTT53450.054.3414883
GTTTCCG458900.053.377036
CGTTTCC430350.052.704815
ACGGTTC45150.052.5598954
AGTTCAC230000.052.331126
CAGTTCA192950.051.974435
GGCGGTT49050.051.765763
GCGGTTC61200.051.0289574
GCCGATT24200.050.402022
CCGTTTC422800.048.8760074
TCGGTTC86850.048.4179234
Produced by FastQC (version 0.11.5)