FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6880103
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT583090.8475018469926977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT362140.5263583989948988No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270790.3935842239571123No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148090.21524387062228575No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG134670.19573834868460543No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC132540.19264246480030894No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87620.12735274457373677No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG83420.12124818480188451No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC82060.11927147020909426No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC78820.11456223838509395No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG73880.10738211332010582No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA73510.10684433067353787No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG73310.10655363735106872No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG72020.10467866542114268No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA70870.10300717881694503No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT69550.10108860288864863No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94550.085.921831
GTATCAA246550.060.2520681
ATCAACG319500.049.212143
TCAACGC321400.049.050484
CAACGCA322400.048.879175
AACGCAG338200.046.5533336
GCAGCGC16800.045.686698
TGCAGCG17200.044.280227
GCTCTAG16000.041.661071
ACGCAGA381400.041.232987
TATCAAC386100.040.8949662
CGCAGAG387100.040.624068
GCAGAGT410550.038.2601979
TGGTATC30100.036.1840362
GTGGTAT32250.033.5872231
TGGTCCT32200.031.788755
CGTCAAT6450.030.4430926
ACGGGAC8700.030.0984213
CTAGCCT56750.028.9434534
CATGCAG28600.028.5072525
Produced by FastQC (version 0.11.5)