FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6880103
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG127840.1858111717222838No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC113210.16454695518366513No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA106810.15524476886465216No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC102070.1483553371221332No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA98350.14294844132420692No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG84350.12259990875136607No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG79920.1161610516586743No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT72060.10473680408563651No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA57600.041.9267355
GGTATCA31600.041.655091
GCTCTAG17050.040.17321
TCAACGC61300.039.6811264
ATCAACG63300.038.6282123
ACGCAGA63250.037.723917
CGCAGAG65200.036.5073288
GTACTTT57600.035.6746371
AACGCAG68600.035.2989776
GTATCAA74200.034.8376431
GCAGCGC13750.034.6222238
TGCAGCG14400.032.2303477
GCAGAGT74150.031.9454579
TGGTCCT36200.029.2481865
CTAGCCT59100.029.0824874
ACGGGAC9700.026.9865553
GCCTTTC56000.026.0322047
GTCGTGC8200.023.9697251
CTTTCTA63850.023.6760929
GAGTACG69600.023.34754412-13
Produced by FastQC (version 0.11.5)