FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16996509
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC431930.2541286566553167No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC381330.22435783724763714No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG247170.145423980889252No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC202870.11935980500466302No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCG12250.0206.3567594-95
ATCGGAT18100.0150.4045394-95
ATCGGAC22450.0138.5846394-95
CCTAGCG10900.0133.7969494-95
AGTCACG20800.0121.5322194-95
GAGCACG31350.0114.7481294-95
ACGTCTA22600.098.9465894-95
GATCGGT21600.094.52505594-95
AAGAGCG40500.091.2241894-95
ATCGGAG40000.085.0725594-95
CCAGTCG22100.079.1884194-95
GTCACCG23700.077.9447194-95
TCGGAAT25150.077.3167194-95
TCGGAAC27000.075.6200494-95
AGATCGT33950.074.45862694-95
GAACTCG24100.072.6167694-95
ACGTCTC19300.070.5264794-95
CGGAAGT41500.065.59811494-95
AGATCGA55250.065.1104794-95
TCGTATC12102.8921932E-1064.281594-95
Produced by FastQC (version 0.11.5)