FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16996509
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG176430.10380366932997828No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTGC301350.056.618926
GTGCTCT419900.055.4244549
TGTGCTC424250.054.251558
GTGTGCT430100.053.750487
CGGTGTG103900.053.5206385
CCGGTGT61700.046.216664
TCCGACC60700.039.5763559
GGGTGTG221000.039.503435
GCGGTGT38300.039.27084
TTCCGAC61600.038.8437588
CGGGGAT160250.036.5288938
CGGGTGT59250.036.0357364
TCGGGGA165750.035.570577
CCGATCT347650.034.2642316-17
TTCCGAT358000.033.67767714-15
TCCGATC343600.033.1959516-17
TGTCGGG40600.032.0209165
CGTGTGC62200.031.8859216
CTTCCGA367500.031.5211814-15
TTTCCGA78350.031.0814427
Produced by FastQC (version 0.11.5)