FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12453809
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC312900.25124843331064417No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC272910.2191377754388236No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGTA7500.0116.2978694-95
ACGTCTA17550.0115.9665294-95
CACGTCA26050.0115.3305794-95
CACGTCG9350.0103.65227594-95
AGTCACG13700.099.03709494-95
TCGGAAT19000.096.9148894-95
GAGCACG24700.094.168394-95
GATCGGC22500.090.4538894-95
GATCGGT15250.088.9710394-95
AAGAGCG31750.088.5206794-95
CCAGTCG19100.081.18523494-95
ATCGGAC15750.073.83990594-95
CTGAACG26150.070.4161694-95
ATCGGAT15600.068.3374294-95
ATCGGAG25400.064.8642994-95
CGGAAGT27100.064.37150694-95
AGATCGA49700.056.5499394-95
CGGAAGC38950.052.25192394-95
ACACGTA27900.052.10477494-95
TCGACCG16300.048.9659543
Produced by FastQC (version 0.11.5)