FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15887249
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC528610.33272594896699864No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC410830.2585910247897544No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG199310.12545280809786516No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC194870.12265811406367458No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCG12150.0142.7559894-95
GATCGGT21000.0142.2461294-95
ACGTCTA23050.0112.87321594-95
ATCGGAT18900.096.87012594-95
GAGCACG29750.084.2140394-95
ACGTCTC19750.082.9430294-95
ATCGGAC19200.075.2814794-95
GAACTCG23050.075.2488194-95
CACACGA35300.070.9735894-95
AACTCCG29050.069.65803594-95
TCGGAAT20850.069.32394494-95
AAGAGCG42050.064.163894-95
AGATCGC42450.061.28922794-95
CTATGCG15604.7293724E-1155.5924794-95
GTGCGTA10555.5631426E-754.8020694-95
AGATCGT32250.053.78248694-95
AGATCGA55650.053.67778894-95
CGTCTGC27550.048.96711394-95
CATAGCG10052.6760641E-547.9404494-95
CACGTCA30300.047.7031194-95
Produced by FastQC (version 0.11.5)