FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13657577
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC616030.451053653221212No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC549320.40220897162066155No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC324310.23745793269186766No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT183350.13424782448599779No Hit
AGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAA169350.1239971043179914No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT157750.11550365046450041No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCG7503.6379788E-12102.5072794-95
GTGCGTA12250.094.1393294-95
GATCGGT14400.093.431194-95
ACGTCTA16550.081.29352694-95
ATCGGAT13550.078.0152194-95
GATCGGC19100.075.4716594-95
ATCGGAC13000.073.923594-95
TGCGACA81350.071.47624
GCGACAT85050.068.421275
AAGAGCG40200.066.9357194-95
CTGCGAC88400.065.8283843
CGACATC89900.064.833736
GCTGCGA92350.062.014142
TCGGAAT20750.060.2075894-95
AACTCCG21200.058.92959294-95
ACGTCTC14251.366061E-953.95119594-95
GGCTGCG114150.050.694211
CACACGA26550.050.67449694-95
AGATCGC34300.050.4317894-95
TCGGAAC17501.5279511E-1049.42314594-95
Produced by FastQC (version 0.11.5)