FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13657577
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG274320.20085553974910778No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA168870.12364565105508832No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG142570.10438894102519064No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGG37150.046.1046455
TCCGACC58000.045.757129
TTCCGAC62950.042.0836378
CGGGGAT192500.041.4090048
TCGGGGA197100.040.606287
CTGTCGG42350.039.5473674
CCCCCGT33850.036.4526181
GTCGGGG50650.036.1587756
TTTCCGA77600.034.562817
TCTGTCG48000.034.494533
CCCCGTT42600.034.3010332
CCGTTTA26000.033.5769164
ATCGGGG146300.033.3391576
GTTTCCG68150.032.721856
GTTCCGT24000.032.6277241
TCACCCC206150.032.6102949
TCCGTTT83300.032.1787763
CCGTTTC80600.032.0817264
TTCACCC213400.031.2131868
CTCCGTT62650.031.0476592
Produced by FastQC (version 0.11.5)