FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17175726
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC407500.2372534354588563Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTGC483950.053.0795636
CGGTGTG171050.051.1773535
GTGTGCT774300.050.186397
TGTGCTC787250.048.9616938
CCGGTGT93150.048.74544
GTGCTCT815950.048.0953879
GGGTGTG303800.039.9500545
GCGGTGT50550.038.7644354
CGGGTGT68550.038.6216934
CGTGTGC100650.037.42976
ATTGCGC61150.033.772599
CCCGGTG56250.031.7129733
ACGGTGT52250.031.6915654
GTCCCGT25750.030.9619061
TCCGGTG41150.030.8984433
ACCGGTG30200.030.6336923
CCCCCGT33600.030.5077461
CATTGCG68950.030.2271218
GGGGTGT135950.029.6300954
CCGATCT701200.028.93864616-17
Produced by FastQC (version 0.11.5)