FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17173844
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG325710.18965468651048653No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA243560.14182031698902123No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA240090.13979980253692767No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG181070.10543358842667955No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG176310.10266193171429763No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC78750.044.316798
TGTCGGG42800.042.767565
TCCGACC86650.039.9969949
CGGGGAT232150.039.5286038
GTAGCAA245250.039.0408139
TCGGGGA237200.038.7872627
CTGTCGG47750.036.844724
TCACCCC276150.036.4110959
TAGTAGC257700.036.402867
GCCCCGT47300.036.3913461
AGTAGCA266250.035.8053258
TTTCCGA100300.034.700287
TCTGTCG50300.034.598723
GTTTCCG86850.034.1560756
GTTCCGT24100.033.5415081
GTCCCGT28450.033.4270821
GTCGGGG58500.033.236916
ATCGGGG175200.032.534066
TCAGTCG188600.032.45855784-85
AGTCGGT189300.032.1251986-87
Produced by FastQC (version 0.11.5)