FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13881657
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG212540.15310852299548966No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA208420.15014057759819308No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG157120.11318533515127192No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA157080.11315652014741467No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGG32550.045.91845
GTAGCAA229150.041.5600669
CGGGGAT161650.040.353048
TTCCGAC53150.039.480648
TAGTAGC236000.039.2646457
TCGGGGA167300.039.1009377
AGTAGCA244450.038.900588
CTGTCGG39900.037.5786674
TCCGACC57200.036.8512429
GTCGGGG45200.035.482176
GCCCCGT34950.035.308571
TCTGTCG43850.033.7585643
CCGTTTC67700.033.5016674
TCAGTCG179750.033.29249284-85
AGTCGGT180450.033.05277386-87
AAGGCCG197550.032.87319636-37
AAACGAG200250.032.73803722-23
ACGAGAA200100.032.6675724-25
CCCCCGT32750.032.4631541
GTAGTAG179750.032.0993736
Produced by FastQC (version 0.11.5)