FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18990766
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG403060.21223999074076316No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA344230.18126177743435942No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG246750.12993156779457976No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG223700.1177940900330192No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA194730.1025393077877954No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC123450.048.2954869
TGTCGGG52000.048.0012635
TTCCGAC130050.045.9958348
CTGTCGG58250.041.9589464
CGGGGAT290550.040.291688
TCGGGGA294700.039.8049627
GGTGTGC149300.039.7383886
GTCGGGG67400.039.3577086
GTGTGCT191300.039.1235247
TTTCCGA157950.038.112057
TCACCCC419750.037.910999
GTTCCGT34700.037.547771
GTGCTCT199700.037.4975289
GTTTCCG135650.037.1835676
TCTGTCG66300.036.2188953
GTTCACC450450.035.3311167
TTCACCC454700.034.990428
CGGTGTG56750.034.767945
CCGTTTC148550.034.2186164
TGTGCTC221300.033.8198368
Produced by FastQC (version 0.11.5)