FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12417805
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC311370.25074479749037776No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC288770.23254512371550365No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT185340.14925343085996276No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC135220.10889203043533055No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCG6350.0197.637294-95
ACGTCTA13900.0118.0682794-95
ATCGGAT11650.082.8653994-95
TGCGACA49750.068.002814
GCGACAT53000.063.9203835
ATCGGAG18550.062.45057794-95
CTGCGAC55700.060.988553
GCTGCGA62500.052.6506922
GTGCGTA11057.5584103E-752.41892294-95
GTCGAAC5550.00711897952.18279694-95
CTGAACG31500.052.0999794-95
GTGCGCA16901.03682396E-1051.41086694-95
CACGTCA23100.050.14970494-95
TCGGACA13856.405935E-848.7918694-95
CTAACCG8205.620198E-447.09179794-95
ATTAACG11156.4446795E-943.2906694-95
TTAGGCG13553.0807223E-642.7475394-95
ACCCGAC44550.042.0848773
CGACATC81650.041.7760166
AGATCGA42150.041.2262794-95
Produced by FastQC (version 0.11.5)