FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17612144
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG334900.1901528854181524No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA199190.11309809867555023No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG185810.10550106789951298No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTGC342350.055.1518026
GTGTGCT492200.052.8797657
CGGTGTG119600.052.1610645
GTGCTCT507650.051.186299
TGTGCTC507800.050.984318
CCGGTGT71450.044.3533174
TGTCGGG47100.042.4046065
TCCGACC80100.040.86889
TTCCGAC80600.040.6741338
CGGGGAT227350.040.567358
GGGTGTG230650.040.2521635
CTGTCGG50050.039.052164
TCGGGGA238750.038.769417
GCGGTGT40600.037.625454
TCTGTCG53350.036.2796253
CGGGTGT62100.036.134454
TCACCCC284450.036.009919
GTCGGGG63500.033.992626
CGTGTGC64850.033.9433676
GTTCCGT28550.033.7380181
Produced by FastQC (version 0.11.5)