FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18305994
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC774450.42305815242810635No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC554900.30312475793447763No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC387890.21189234520671207No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA84400.067.294024
GCGACAT87950.064.631155
CTGCGAC96000.059.4039423
GCTGCGA102950.055.2181972
CGACATC114000.050.1060566
GGCTGCG127200.045.603791
ACCCGAC41200.032.6083763
ACATCTG198350.029.1016648
GACATCT200850.028.69337
CTAGGCG284200.028.461137
TAGGCGG290650.027.7976538
CCCGACT49350.027.6925724
TGGCTAG300650.027.3813384
CCGACTC49850.026.7645845
GCTAGGC304950.026.6308636
ACTCGGG122300.026.5400851
CATCTGT219750.026.0779829
GGCTAGG316850.025.8354475
TTGATCG112950.025.3682322-23
TGATCGC114750.024.88955324-25
Produced by FastQC (version 0.11.5)