FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18305994
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG321300.17551628171625097No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA218490.11935434918202202No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC57500.042.744328
TCCGACC58100.042.139579
TGTCGGG44650.041.8664675
CGGGGAT215900.039.514238
TCGGGGA222900.038.486187
CTGTCGG54950.033.846294
GTCGGGG57900.033.5148666
CCCCGTT51050.032.5283972
GCCCCGT43250.032.3693731
CCCCCGT35800.032.0797351
TCTGTCG56800.031.9913433
GTCCCGT30800.031.7405831
ATCGGGG162400.031.552266
TTTCCGA81550.030.7203677
CCGTTTC80950.030.5361024
ATAGCGC109850.030.32920394-95
CCGATCG120100.030.1874686-87
TCACCCC208750.029.980229
TCTATCG123050.029.5730273
ACCCGAT123200.029.44348584-85
Produced by FastQC (version 0.11.5)