FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22991182
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG1497130.651175742073635No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT890560.3873485060489713No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC805310.3502690727253605No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT734980.31967908392008726No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC646620.281246958072882No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG635600.2764538160760939No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG558300.24283223020025677No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA514240.22366836120039413No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA502520.2185707546484561No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC490510.2133470127808131No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA371700.16167067878458793No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT352150.1531674187086162No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC351500.15288470162169132No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT311930.1356737552684329No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT311380.135434533117958No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC304120.1322768007316892No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT300860.13085886580341977No Hit
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG293800.12778812329005096No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC270980.11786257879216476No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG246690.10729765872846381No Hit
GCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGG235600.10247407027616066No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA60500.059.6311654
GCGACAT65350.055.351115
CTGCGAC72600.050.150443
GCTGCGA96500.037.778952
ACCCGAC244450.036.6633
CCGACTC252300.035.5787965
CCCGACT253150.035.459184
CGACTCC256550.034.8795176
AACCCGA261550.034.4111822
GACTCCC264650.034.0272257
CCCTTAG211700.033.320693
TAGAGCC209050.033.3127947
ACTCGGG167000.033.2353321
ACTCCCT278850.032.277428
TTAGAGC219050.031.7703386
GCCCTTA225150.031.3934292
GAACCCG299500.030.3739051
GTATTCG172450.030.0925481
GGCTGCG124500.030.0509931
CTCGGGA186150.029.7338732
Produced by FastQC (version 0.11.5)