FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19292279
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC915230.47440222070186727No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC712200.3691632284604634No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC436130.22606453078975272No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT386570.20037549736866234No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT248590.12885465734763632No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA107550.066.869934
GCGACAT111000.065.005365
CTGCGAC119950.060.273913
GCTGCGA128400.056.4921462
CGACATC141100.051.0723426
GGCTGCG158300.046.3989141
GACATCT219150.033.316237
ACTCGGG214100.032.1329151
ACATCTG229950.031.772128
ACCCGAC43400.030.735933
CTCGGGA224700.030.4432242
CTAGGCG354200.029.4457427
TGGCTAG360400.029.188624
TCGGGAG235900.028.9978493
TAGGCGG363900.028.6869378
CATCTGT260250.028.1642079
GCTAGGC374750.027.9703856
TTGATCG133800.027.24807722-23
GGCTAGG386250.027.2351595
GCGCGGT68800.026.8412156
Produced by FastQC (version 0.11.5)