FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19292279
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG350640.18175146648045054No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA286470.1484894552893414No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG220120.11409745836663465No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC107900.050.80339
TTCCGAC115350.047.8578038
TGTCGGG47200.045.6297535
TTTCCGA135200.041.2885447
GTTCCGT36200.039.2763561
GTTTCCG122250.039.0043956
CGTTTCC124450.037.935545
CGGGGAT260800.037.666988
CCGTTTC127300.037.539674
TCGGGGA265200.037.2392657
CTGTCGG56800.036.918414
TCCGTTT129600.036.1029663
GTCGGGG61900.035.9431956
TCACCCC346900.034.605189
CCCCGTT57600.033.7639772
TCTGTCG61300.033.5876923
CTCCGTT103800.033.3493042
GTCCCGT32550.032.431761
GCTCCGT91700.032.4100151
ATAGCGC204700.032.14631794-95
Produced by FastQC (version 0.11.5)