FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12268255
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT622020.5070158714503408No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG481380.39237854120247745No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC376230.30666953042629125No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC359730.2932201849407271No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT356920.2909297206489431No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG327040.2665741786423579No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC312700.2548854747476312No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC271530.22132731998152957No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC210410.17150768385560947No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG201160.16396789926521743No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA193440.15767523580166862No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC180120.1468179459915041No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA147230.12000891732361285No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT140570.1145802724185306No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG125750.10250031483695114No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA42900.064.8982854
GCGACAT44150.063.1657835
CTGCGAC47700.058.561883
GCTGCGA57400.048.9138342
GAACCCG137600.047.9531251
ACCCGAC139400.046.723853
CCGACTC141550.046.1450735
CCCGACT142600.045.8052944
CGACTCC144800.045.1411636
GACTCCC148550.044.0951737
AACCCGA147950.044.0605662
ACTCCCT157650.041.814348
GGCTGCG73300.039.3474771
CTCCCTT178150.036.9505429
CCCTTAG102050.032.1846853
TAGAGCC103450.031.3907597
CTAGGCG144850.030.2542887
TTAGAGC108300.030.0705386
GCCCTTA110850.029.8841762
TAGGCGG147800.029.619098
Produced by FastQC (version 0.11.5)