FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8360054
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT646510.7733323253653624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT356770.4267556166503231No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352320.4214326845257219No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG154640.1849748817411945No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT140630.16821661678261887No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC116940.13987947924738287No Hit
TAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTA111740.13365942373099504No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC108940.13031016306832469No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG99410.11891071517002162No Hit
GGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAG93660.11203276916632357No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA92610.11077679641782218No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA89120.10660218223470805No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC85790.1026189543751751No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC85710.1025232612133845No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG85710.1025232612133845No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC84400.10095628568906373No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111900.089.194821
GTATCAA331900.064.806341
GCTCTAG14950.060.5116541
GCAGCGC26000.050.344678
ATCAACG432500.050.240273
CAACGCA441100.049.3821835
TCAACGC440400.049.339054
TGCAGCG27450.047.685587
AACGCAG464250.047.0118756
TATCAAC502750.043.1264572
CATGCAG30550.043.0533875
ACGCAGA509800.042.762437
CGCAGAG513700.042.4259458
GCAGAGT542650.040.1406179
GTACTGG21550.032.8652041
CAGCGCA46150.032.101989
ACGGTAC9350.031.8261893
AGAGTAC604100.029.3994810-11
GTCGTGC12850.028.252921
CAGAGTA585200.027.3191710-11
Produced by FastQC (version 0.11.5)