FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17531043
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT935610.5336875849314842No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC856040.4882995267309538No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG636040.36280784891121426No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC597240.3406756802775511No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG511810.2919449801132768No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC460490.2626711941782357No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC424800.2423130215355698No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC415450.23697962522823088No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT412010.23501739172050404No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC332090.18942968766889684No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA325730.18580183734647163No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG295610.16862088581951457No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT282060.16089173929925332No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA231560.1320856950724495No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT222450.12688919877727753No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT200620.11443700183725519No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG192280.10967972641445235No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT190230.10851037214385933No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA188660.10761481789760027No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA88750.069.791344
GCGACAT91050.068.232455
CTGCGAC96650.064.134653
GCTGCGA111700.055.2904472
GAACCCG204000.047.530651
ACCCGAC213950.044.649323
GGCTGCG142900.044.06741
CCCGACT218150.043.8321654
CCGACTC220850.043.1498265
GACTCCC224000.042.6261257
AACCCGA224900.042.6024172
CGACTCC224150.042.5149276
ACTCCCT234600.040.581658
CTCCCTT262600.036.307629
ACATCTG179450.035.007588
CCCTTAG138650.032.0098843
CATCTGT201350.031.2920789
TAGAGCC143350.030.5730927
CGACATC205800.030.1876496
TGGCTAG307300.030.1060644
Produced by FastQC (version 0.11.5)