FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17531043
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA730450.4166608911974034No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG513760.29305729271213354No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG349810.19953747190055948No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG275740.15728670564552263No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG258200.14728159642298522No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG256640.1463917463438998No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA244000.13918167903643838No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA242490.13832034979322108No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA235920.13457271196014978No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG227710.12988959071060405No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG213210.12161854830884848No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG185220.10565258438987343No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG176830.10086678813120246No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGT20350.049.658512
GTAGCAA572000.048.732799
TGTCGGG43150.047.7189755
GCTAGTA56150.047.655345
AGTAGCA613800.045.352368
GCCCCGT54350.044.9655951
CGACCTA13300.044.5996061
TTCCGAC94600.044.538198
TAGTAGC640150.043.085317
GTGCTCT341700.041.7513586
GATAGTA87750.041.361525
CGCGATA5200.041.0565723
GCTCTGA397100.040.4812748
TTTCCGA104550.040.435647
GGTGTTA78150.040.0676082
CCCCGTG55650.039.813482
CCGTGCT44200.039.3936274
GGTACGG26800.038.9547651
GTAGTAG444600.038.8004346
TCTGTCG51500.038.6914983
Produced by FastQC (version 0.11.5)