FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16263038
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG398840.2452432319225965No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA241740.14864381427381526No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG218030.13406474239314942No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG183500.11283254703088069No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC93250.049.0998239
TTCCGAC93650.049.0927628
TGTCGGG53300.048.5099335
CTGTCGG60650.041.692744
TTTCCGA111450.041.635297
TCTGTCG61150.040.7296683
GTCGGGG66650.040.644586
CGGGGAT273500.040.628528
TCGGGGA276050.040.5111547
GTTTCCG98650.039.002326
GTTCCGT26350.038.3598251
CGTTTCC97800.037.8855445
TCACCCC308400.036.2923479
CCGTTTC105600.036.165634
CCCCGTT53350.034.6802442
TCCGTTT106000.034.148113
GTAGCAA152950.032.540749
GCCCCGT42700.032.00681
CTCCGTT86800.031.9733522
ATCGGGG204850.031.798116
Produced by FastQC (version 0.11.5)