FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17024513
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC510200.299685518170182No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT340760.2001584421240126No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC331240.19456650536787748No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC268050.157449437760716No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG199460.11716047325406606No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT188420.11067570625955644No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG174400.10244052208718099No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA54250.059.4348874
GCGACAT60100.053.6508945
CTGCGAC65050.049.8520163
GCTGCGA72100.044.917282
ACCCGAC86550.038.431683
CCGACTC89900.036.536655
CCCGACT91300.036.1277734
GAACCCG99950.034.8425751
CGACTCC95500.034.44286
GGCTGCG100950.032.7059481
AACCCGA107300.031.175182
CGACATC105550.030.8121746
CTAGGCG185350.028.844087
TAGGCGG190850.028.2313088
GACTCCC119150.027.8006847
GCTAGGC205150.026.3989376
TGGCTAG208100.026.2689024
GGCTAGG215000.025.2756165
ACTCGGG127850.025.24421
TCGATCG66600.024.9718616-17
Produced by FastQC (version 0.11.5)