FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15308331
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC782830.511375145990768No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC583720.38130871353643975No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC350750.22912360596331502No Hit
AGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAA199080.1300468352820435No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT186870.1220707861621231No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT162930.10643224267883938No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA84150.067.234064
GCGACAT87500.064.8188255
CTGCGAC94600.060.1009333
GCTGCGA101600.056.342462
CGACATC106350.053.4195946
GGCTGCG126600.045.880921
GACATCT179950.031.7768557
ACATCTG183050.031.415918
CTAGGCG277100.029.1647057
TGGCTAG282350.029.1134434
CATCTGT201500.028.539099
TAGGCGG283950.028.3795388
ACTCGGG137250.028.0560951
GCTAGGC290050.027.9744076
GGCTAGG300200.027.2588655
TTGATCG105900.026.99841322-23
CTCGGGA149150.026.0736142
TGATCGC111000.025.71628224-25
GGCTGGC322100.025.5663721
CTGGCTA327050.025.3468463
Produced by FastQC (version 0.11.5)