FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17522866
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA385310.21988982852462605No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG314430.17943982451272525No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG263170.15018661901540536No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC79000.044.7347228
GTAGCAA360200.043.7239
TGTCGGG39100.042.095775
GCCCCGT46700.041.7472721
AGTAGCA387850.040.6059658
TAGTAGC390200.040.0186167
TTTCCGA89750.039.744127
TCCGACC90900.038.773999
CGGGGAT183800.037.913328
GTTTCCG79250.037.5255246
CCGTTTC84700.037.465454
CGTTTCC80850.036.6678775
TCGGGGA191100.036.5124937
GTTCCGT24150.035.7484661
GTAGTAG285950.034.202446
TCAGTCG295150.034.1471984-85
AGTCGGT293800.034.04746286-87
AAACGAG320550.033.864322-23
ACGAGAA322600.033.55995624-25
TCCGTTT88350.033.5001263
Produced by FastQC (version 0.11.5)