FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16629450
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA331780.1995135136760386No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG268640.16154472938070713No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG238190.14323384116732665No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC67900.045.9676068
TGTCGGG31250.045.0763365
GCCCCGT38800.044.3741571
GTAGCAA326750.043.3045359
TAGTAGC349750.040.1699147
AGTAGCA355550.039.7301068
TCCGACC78750.039.5740559
TTTCCGA79850.039.0862967
CCGTTTC78300.037.4949154
GTTTCCG71700.037.1750456
CGGGGAT174600.036.8664558
TCGGGGA176400.036.48847
CGTTTCC76650.034.9605675
CCCGTTA19300.034.6476633
TCCGTTT82150.034.5227783
GTAGTAG259500.034.4515156
TCAGTCG262700.034.3162484-85
AGTCGGT262750.034.04297386-87
AAACGAG286450.033.84517322-23
CTGTCGG41250.033.8039054
Produced by FastQC (version 0.11.5)