FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8475753
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG141390.16681703678717394No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT135770.160186357483518No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC109490.12918026280378864No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA106270.1253811903201993No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC106220.12532219851144788No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG102490.12092140957859437No Hit
GGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAG95960.11321707935566315No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG93290.11006691676833905No Hit
TAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTA91980.1085213313790527No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT85570.10095858149712479No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTAG17350.064.182211
GGTATCA39650.061.876621
GCAGCGC25050.050.5852288
CAACGCA118250.049.6207735
TGCAGCG25800.049.342697
ATCAACG119900.048.5408553
GTATCAA128050.047.5273781
ACGCAGA124950.047.0380677
CGCAGAG126500.046.3701258
TCAACGC125400.046.364144
AACGCAG133500.044.0249946
GCAGAGT134700.043.331079
CATGCAG32600.039.794835
GTACTGG22950.039.1802141
CAGCGCA46400.030.1333839
TATCAAC210350.028.375342
GTCGTGC14400.027.7067031
GAGTACG150350.026.59671212-13
TAGATGG28450.026.555247
GGTTCAC33600.025.6715666
Produced by FastQC (version 0.11.5)