FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19405359
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA321220.165531593618031No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG300070.15463254248478475No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG278360.1434449112742516No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCAA389500.044.8290829
TAGTAGC410100.042.2164967
TGTCGGG42300.041.823965
AGTAGCA421600.041.3367848
TTCCGAC62650.041.1127858
CGGGGAT192500.039.6235548
TCGGGGA197600.038.551187
CCCCCGT45000.036.0493281
GCCCCGT45950.035.8201641
CGACCTA14050.035.7861
TCCGACC73150.035.1468549
TCAGTCG315850.034.9229184-85
AGTCGGT319500.034.57486786-87
GTAGTAG309600.034.530866
ACGAGAA346800.034.12569424-25
AAACGAG348500.034.0218522-23
CTGTCGG52000.033.8398484
AAGGCCG351050.033.71891836-37
TCGGTCC324550.033.52886288-89
TTTCCGA78400.033.0335857
Produced by FastQC (version 0.11.5)