FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19133750
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT543320.2839589730188803No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG486890.25446658391585547No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC477180.2493917815378585No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT362940.18968576468282486No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC360430.1883739465603972No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG275440.14395505324361402No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC262150.1370092114718756No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC221250.11563337035343307No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC192790.10075912980989091No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCG9058.947609E-8273.7837294-95
GTTCGCG5100.0015130634242.9159994-95
GAACTCG20703.063724E-8149.6221694-95
GAGCACG29501.720764E-9125.9869394-95
GTTTCCG17704.4391272E-4104.9891194-95
ACACGCA30453.0627052E-7101.71358594-95
ATCGGAG25101.43706675E-598.7148794-95
ACGTCTC19156.071113E-497.0395494-95
CGTCTGC25951.6955082E-595.4814394-95
CCAGTCG20307.6548016E-491.5422294-95
AATAGCG13704.82396E-990.4285894-95
CACACGA30752.7284841E-1180.57794-95
TTTTACG24350.001576448876.3165294-95
GAACACG34206.667107E-572.4486294-95
CACGTCA44950.068.90275694-95
TTCGTCG9901.5017577E-862.56926794-95
AAGAGCG41301.6959848E-459.99378294-95
TCGTACC10550.002259158558.71428394-95
TGAACCG33000.0052589956.3123494-95
TGCGACA52550.052.686484
Produced by FastQC (version 0.11.5)