FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19133750
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA358300.18726073038479127No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG320220.16735872476644673No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCAA372350.043.810329
GCCCCGT44650.041.9787371
AGTAGCA402500.040.6111538
TAGTAGC398550.040.5136227
CGGGGAT128050.034.6460728
TCAGTCG301750.034.15922584-85
GTAGTAG296550.034.1443186
CGGTGAA88100.034.0938345
AGTCGGT303050.033.82710386-87
TCGGGGA133000.033.463647
AAACGAG330550.033.4023222-23
AAGGCCG327950.033.3987836-37
GTTCCGT24150.033.206521
ACGAGAA332450.033.18901424-25
TCGGTCC306000.033.00548688-89
GTACGGT18800.032.0557022
CGAGAAC322350.031.99162724-25
CCCGCGA68000.031.74485264-65
CGAAGTG318500.031.68083640-41
CAGTCGG308450.031.67063784-85
Produced by FastQC (version 0.11.5)