FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17333801
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC1618550.9337536527620226No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC956020.5515351191582274No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC587260.3387947052120882No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT370060.21349039371110815No Hit
AGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAA293870.1695358104088076No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG292430.16870506359222653No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT226210.130502248179727No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG224770.12967150136314592No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC193990.11191428815872524No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG187700.10828553991129816No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCG10652.0270818E-7232.3570394-95
GCGCTAG7700.005210207160.6884894-95
GAGCACG26101.23051E-7118.5154494-95
ATCGGAT16153.097862E-4114.9196194-95
ACGTCTC18755.6094513E-498.984194-95
TCGGACA19156.1005127E-496.9165494-95
CCAGTCG26251.8057888E-594.2705694-95
CACGTCA35057.128583E-788.2525894-95
TACACCG14551.8464574E-585.0378894-95
ATAGACG22050.001068319884.1701594-95
ATCGGAG28400.002915978765.3504294-95
TGCGACA145400.065.072874
AACTCCG28550.002977497665.0070794-95
GCGACAT147350.064.274665
CTGCGAC157400.060.082323
GTGCGTG52808.128987E-658.5843494-95
GCTGCGA162850.058.3135572
GCCCACG33250.005444557455.81809694-95
GTGCGCA24352.6375346E-1050.8131994-95
GGCTGCG193400.049.3617941
Produced by FastQC (version 0.11.5)