FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17142179
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT1097330.6401344893201734No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT903830.5272550239966576No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG895050.5221331547173788No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC875800.5109035438260212No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC742980.4334221454577041No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG578730.33760585512495234No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC527090.3074813301156172No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC362980.21174670968025713No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC289500.16888168067781814No Hit
CCCTCATGCTGCCACCCCCGACTGTCACTGCACCGACCGGCCCTCGAGGA287820.16790164190911785No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC267420.15600117114632858No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT263820.15390108807054226No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA224820.1311501880828569No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG213100.1243132509583525No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA209180.12202649383138515No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA206570.12050393360144006No Hit
GAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCG202970.1184038505256537No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG201670.11764548719273088No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC175870.10259489181626211No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA175490.10237321638048465No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC173780.10137567691948614No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG171800.10022063122780366No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCG9150.0400.9795894-95
ATCGGAC15101.8189894E-11242.9776894-95
GATCGCG15001.1836E-6163.0650294-95
CGTCTCG7800.005606944156.793394-95
CACACGA28600.0149.6663494-95
GAGCACG26458.8039087E-10138.7131794-95
ATCGGAT13401.543326E-4136.9016194-95
CGTTACG4705.321886E-4130.1050794-95
TGAACCG33200.0128.9294394-95
ATCGGAG26752.1010306E-591.4383494-95
AAGAGCG40351.6581907E-890.9284594-95
ACACGCA27402.366916E-589.2691794-95
TTCTGCG24850.001798271273.822294-95
GGAAGCG42852.5230347E-671.35284494-95
TACCACG18650.065.57574594-95
ACGTCGA9452.1471169E-764.7083494-95
ATACCGC9551.8189894E-1264.0307794-95
TCTGACG58050.063.203594-95
TGCGACA84700.062.197834
TAGGGCG10000.061.14938494-95
Produced by FastQC (version 0.11.5)