FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18325501
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT1354370.7390630138843135No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT1010580.5514610487320374No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC956880.5221576206838765No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG891250.4863441386950349No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC776230.42357914252930934No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG771420.4209543848214573No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC444420.24251451570137156No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC421520.23001826798623404No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC420730.22958717472444548No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT357470.19506697252096955No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC335820.1832528343972697No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA306110.16704045362797992No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG260610.14221166449964998No Hit
GAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCG256780.14012168071148504No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG252800.13794984377234762No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA248950.1358489462307197No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA239780.13084499026793317No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC238920.1303756988690241No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA227830.1243240225737894No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG224090.12228315067620797No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC218720.1193528078713919No Hit
AACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACC206020.1124225744223855No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC205750.11227523875063498No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC198700.1084281406549267No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATTCG9303.351677E-5200.827894-95
TTAGTCG8000.0057322485155.6415694-95
TAAACCG12651.14242255E-4147.6441794-95
CTATACG9300.008991351133.8852194-95
CGTCTGT24107.367453E-8129.1631294-95
GCGTACG5205.182086E-4119.7242894-95
GAACTCG23409.890686E-6106.42158594-95
ATCGGAG29902.665529E-7104.1080794-95
TACACCG13151.0604708E-994.6868994-95
GAGCACG31504.3251843E-579.0560494-95
ACATGCG26650.002210345870.082594-95
GACGCGA9154.064248E-668.0400294-95
CCTAGCG9250.003254199967.3044694-95
ATAACCG9500.065.5332994-95
CAAAACG28650.002944807365.1901894-95
GATCGCA30550.003797923761.135894-95
CCGTTCT30600.003822603561.03590494-95
CAACGCG10450.059.57571494-95
GCCGTTA21400.058.18375894-95
TGCGACA75250.056.38724
Produced by FastQC (version 0.11.5)