FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18996594
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC486390.25604063549497347No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC392440.20658440139321815No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT320170.16854073946097917No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC209550.11030924806836426No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA79550.064.183284
GCGACAT79950.063.92145
CTGCGAC90700.056.40613
GCTGCGA96250.052.0763132
CGACATC116300.044.065166
GGCTGCG122350.041.3573231
ACCCGAC84650.038.6642763
CCCGACT90550.036.7166634
CCGACTC92350.035.2809035
GAACCCG103800.032.9107931
CGACTCC99200.032.7968756
AACCCGA109300.030.4275072
GACTCCC122000.026.9016367
TTGATCG98500.026.06348822-23
GACATCT198800.025.9939927
GGTGTGC197350.025.7277037
TCGATCG65450.025.43501316-17
TGATCGC101350.025.2370124-25
TTCGGCT101650.025.18754840-41
ACATCTG208400.024.8877358
Produced by FastQC (version 0.11.5)