FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7772162
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG100750.12962931035148262No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT93580.1204040780416054No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA80830.10399937623533839No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39150.062.824221
ATCAACG111500.047.8285523
CAACGCA111650.047.477875
TCAACGC113350.046.731384
GTATCAA121800.045.618731
ACGCAGA116950.045.5018967
CGCAGAG117600.045.1236958
AACGCAG125750.042.268756
GCAGAGT133100.039.602999
GCTCTAG19650.038.490181
GCAGCGC23250.036.8456848
GTACTTT83600.036.6867941
TGCAGCG26450.033.0444347
TACTTTT96400.031.4881572
ACTTTTT100650.030.2176883
TATCAAC192050.028.6359622
TAGCGTG11650.026.5552089
CATGCAG35300.026.2919125
GAGTACG140950.026.21563312-13
CAGCGCA35300.025.4492239
Produced by FastQC (version 0.11.5)