FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12148043
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC289370.23820297639710364No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC155110.1276831173547871No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA75900.050.2486044
GCGACAT77550.049.3632165
CTGCGAC81500.046.859813
TCGACCG12250.045.3682333
CGACATC85650.044.5843546
GCTGCGA84950.044.5158162
CCGCTCG12900.042.7097937
CGACCGC15100.038.688194
GGCTGCG103000.036.7170141
GCTCGCG15700.034.7902349
CGCTCGC16550.033.0034268
CTCGACC19050.029.1780032
GACCGCT20500.027.80215
CGCGTCG9900.027.58596812-13
CTCGCGT10200.027.00755110-11
GACATCT144250.026.670337
TCGCGTC10450.026.58869210-11
CCTCGAC21150.026.5071531
GCGCGGT41950.026.153746
GGTGTGC99350.025.815777
Produced by FastQC (version 0.11.5)