FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14959405
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG272130.18191231536281022No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA214850.14362202239995508No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC67000.044.1954849
TGTCGGG38500.043.630665
TTCCGAC68400.043.496128
CGGGGAT212000.038.6317188
TCGGGGA218500.037.6776857
CTGTCGG43000.037.519734
CCCCGTT42300.036.5673142
TTTCCGA86150.034.8095137
GTCGGGG53250.034.5741236
GGTGTGC107450.034.4450846
GTTCCGT25250.034.402961
GTGCTCT141450.033.5478869
TCTGTCG48300.033.3059733
GCCCCGT38050.033.059581
GTGTGCT142600.032.9088677
GTTTCCG75400.032.7872856
TCACCCC225000.032.4792759
CCGTTTC83250.032.4322624
CGTTTCC77150.031.9213055
CGGTGTG41700.031.7480755
Produced by FastQC (version 0.11.5)