FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15669225
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC586280.3741601770349203No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC576510.3679250250092139No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC213290.13612032503202934No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG202180.12902999350637956No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT190470.12155674578672525No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT174590.11142223051873974No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA101400.058.595114
GCGACAT104250.056.902115
CTGCGAC112800.052.808343
GCTGCGA118700.049.5901762
CGACATC135900.043.545516
TCGACCG25750.041.1378633
GGCTGCG145450.040.70081
GCTCGCG26800.040.229719
CTCGACC27500.038.525412
ACCCGAC58800.036.67693
CCTCGAC31150.034.318091
CCGACTC62800.033.9569555
CCCGACT63100.033.8707774
CGACTCC66800.031.9949056
CCGCTCG35200.030.0897147
GACATCT198400.030.0679537
CGTCGTA12900.030.00789838-39
ACATCTG199700.029.8959988
CTCGCGT18050.029.20789310-11
GCGCGGT71200.028.8836926
Produced by FastQC (version 0.11.5)