FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15669225
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA203290.12973838846528785No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG172230.10991609348898876No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGG37200.043.62295
CGGGGAT138250.037.7529228
TTCCGAC46300.037.4054538
TCCGACC47900.036.1588869
TCGGGGA146000.035.878797
TAGTAGC152350.034.5390787
CTGTCGG46750.033.3922464
GTTCCGT25100.032.9069181
CCCCGTT45450.032.6751942
GTAGCAA162900.032.6544389
TCTGTCG48050.032.3914073
GCCCCGT37850.031.3534811
TCAGTCG111600.030.98993984-85
TCGGGTA52500.030.9092487
CCCGTTA21300.030.5204093
CCCCCGT35350.030.2137581
CGGGTAT53750.030.102258
AGTAGCA178150.029.936678
AAGGCCG124150.029.44372736-37
AGTCGGT118450.029.38076686-87
Produced by FastQC (version 0.11.5)