FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23034665
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG462590.2008234111501079No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA261730.11362440044168214No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG240030.10420381629166302No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTGC704650.064.280616
GTGTGCT1012900.061.8964777
TGTGCTC1023450.060.855478
CGGTGTG281550.054.9925585
GTGCTCT1169000.053.956259
GGGTGTG434450.051.5628055
GCGGTGT80950.044.666354
GGGGTGT193650.040.3079574
GTAGCAA609300.039.190019
CGTGTGC139650.038.9042056
ACCGGTG41000.038.3090673
TAGTAGC624450.037.5750587
CGCGATA5850.036.501793
TGTGTGC310300.035.980916
CGGGTGT159550.035.956044
AGTAGCA669000.035.5837638
GTACGGT28200.035.5034072
TACGGTG40400.035.001963
CCGGTGT226350.034.9879264
CCGATCT897850.034.64425316-17
Produced by FastQC (version 0.11.5)