FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20861785
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG520140.2493266995130091No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA493590.23660008000274185No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG286490.13732765436898137No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG274770.1317097266604943No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG252410.12099156424054797No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA232350.11137589616612385No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG218530.10475134318563824No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCAA554850.047.4068539
TTCGACG9450.045.2087141
AGTAGCA595000.044.204818
TAGTAGC588100.044.174487
TCGACGT10650.041.8798682
GCCCCGT50550.041.5061571
CGACCTA14700.040.0421
GTACGGT22700.039.5059932
GTAGTAG442250.037.2736136
CGCGATA5900.036.1932143
AATACCG44250.036.0302778
GCTAGTA65650.035.6331185
GATAGTA101050.035.1711965
TCAGTCG478050.035.06877584-85
CGGTGAA118150.034.9805035
AGTCGGT480850.034.8472386-87
GGTAGTA361650.034.822035
GGTACGG27750.034.554121
AAACGAG516350.034.42078822-23
TCGGTCC481400.034.36802388-89
Produced by FastQC (version 0.11.5)