FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21971726
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC429290.19538292075916114Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC48150.044.544639
TTCCGAC49250.043.450318
GTTCCGT28100.034.4624561
GTCCCGT28400.034.265561
CCCCGTT50500.033.6353842
CTCCGTT60700.032.516922
CCCGTTT64250.031.1665383
GCTCCGT54800.030.9250221
GCCCCGT41550.030.7329061
TTTCCGA71600.030.4836087
TCCGTTT80500.030.4750143
GTTTCCG62550.030.4187646
CCGTTTC81250.029.1998124
CCCCGTA29100.028.3701112
CGTTTCC72950.028.0340235
CCCGTTA24550.027.8330153
TCCCCGT50600.027.6753981
ATTGCGC54500.027.600449
CCGATCT70450.027.54511516-17
TCCGATC66950.027.53231616-17
Produced by FastQC (version 0.11.5)