FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14131506
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT508580.35989087079607796No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT478800.33881739143726086No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC279950.19810344346879943No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC218110.154343068601464No Hit
GAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTAT161990.11463038688162466No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA43500.059.6184464
GCGACAT43850.059.46465
CTGCGAC49400.052.6014333
CGACATC54450.047.801166
GCTGCGA56150.046.366892
ACTCGGG231450.044.0325051
CTCGGGA244800.041.7257582
TCGGGAG253650.040.2849433
GGCTGCG72250.037.023191
CGGGAGG324300.031.929114
TTGATCG50400.026.24592822-23
GCGCGGT41400.025.6982446
CTAGGCG92350.025.0465457
TAGGCGG93600.024.8133688
GGGAGGC459200.024.8132765
GGAGGCT461450.024.7746336
ATTCGGC53600.024.19394338-39
TCGCCAG58100.023.05388326-27
TGATCGC56900.022.99769424-25
TTCGGCT56450.022.97266240-41
Produced by FastQC (version 0.11.5)