FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17701866
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC510860.28859104458253154No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC300290.1696374834155902No Hit
TGGGGGGGATTCTTCTCTAATCTTTCAGAAACTTTGTCTGCGAACACTCT255050.14408085565668613No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT43800.067.2317355
TGCGACA45150.065.433724
CTGCGAC50550.058.9242323
GCTGCGA56350.053.7945332
CGACATC55150.053.6536526
CTAGGCG123300.044.761137
TAGGCGG124750.044.315888
GGCTGCG71950.043.191551
GCTAGGC146250.037.9643026
GTCGGGT21200.037.18948
GGCTAGG163350.034.7751355
TCGGGTT22800.034.3713079
TAACGCG10850.033.27423570-71
AGGCGGG170500.032.647529
CGGTCGA81500.032.20346568-69
TGGCTAG177700.032.047994
TCGGTCG84300.031.66916868-69
ACGCGAC11900.030.73724472-73
TGCGCGC89400.030.52536260-61
TTGATCG49100.030.51969322-23
Produced by FastQC (version 0.11.5)