FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15153515
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC479410.31636884247648156No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC459850.3034609461897124No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT189920.12533065760650253No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA66800.070.319624
GCGACAT68650.068.354535
CTGCGAC73800.063.9164433
GCTGCGA79050.059.9800952
CGACATC82400.057.1212046
GGCTGCG96850.049.0590321
ACTCGGG113300.033.6328051
CTAGGCG161600.031.3305637
TAGGCGG164450.030.8160658
CTCGGGA125800.030.288112
ACATCTG158450.030.1245428
TTGATCG79650.029.54727422-23
TCGGGAG130200.029.333563
GACATCT162300.029.2932767
TGATCGC81000.028.96743624-25
ATTCGGC83700.028.4049538-39
GCTAGGC180900.028.2241466
GGCTAGG189650.027.4228255
TGATTCG85900.027.39879636-37
TTCGGCT88100.026.95935440-41
Produced by FastQC (version 0.11.5)